Item type |
[ELS]紀要論文 / Departmental Bulletin Paper(1) |
公開日 |
2016-07-25 |
タイトル |
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タイトル |
マミズイトミミズの燐脂質区分における燐代謝について(I) : 燐脂質区分の調製 |
タイトル |
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タイトル |
Phosphorus Metabolism in Phospholipid Fraction of Rhizodrilus limasus (I) : Preparation and Purification of Phospholipid Fraction |
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言語 |
en |
言語 |
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言語 |
jpn |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN00039943 |
論文名よみ |
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タイトル |
〓 |
著者 |
大山, 重信
OOYAMA, Shigenobu
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抄録(英) |
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内容記述タイプ |
Other |
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内容記述 |
It was shown previously^<1)-3)> the absorption of ^<32>P and the incorporation of absorbed ^<32>P into various acid-soluble nucleotides when a sludge worm, Rhizodrilus limasus, was exposed to ^<32>P containing water. The present study deals with the preparation of phospholipid fraction from ^<32>P labeled Rhizodrilus limasus and the purification of the fraction by celulose column chromatography. The effectivenesses of extraction for lipid ester and ^<32>P activity from ^<32>P labeled Rhizodrilus limasus with CH_3OH : CHCl_3 (1 : 2) or (1 : 1) solvent were compared each other and no significant differences were observed between the effectivenesses of the two (Table 2). Phospholipid fraction obtained according to Table 1 was passed through cellulose column (Fig. 2) and the atomic N/P ratio of the fraction reduced from 1.07 to 0.85. 26% of lipid ester (E_<510>) and 82% of ^<32>P activity which existed in CH_3OH : CHCl_3 (1 : 1) extract were found in phospholipid fraction, respectively (Table 3). |
書誌情報 |
巻 22,
p. 1-8,
発行日 1972-01-20
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表示順 |
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内容記述タイプ |
Other |
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内容記述 |
3 |
アクセション番号 |
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内容記述タイプ |
Other |
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内容記述 |
KJ00000066043 |